Composite

Part:BBa_K4227023

Designed by: Yang Chen   Group: iGEM22_ZJUT-China   (2022-10-12)


PnlmC-deGFP

PnlmC is derived from Streptococcus mutans ATCC25175, a gram positive bacterium. According to study done by Liu et al.Liu, T., et al., ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans. Annals of Microbiology, 2014. 64(1): p. 31-38., nlmC promoter can sense competence stimulating peptide (CSP).As a result, the main function of this promoter is to sense a quorum sensing chemical, competence-stimulating peptide (CSP), and trigger down string gene.

The deGFP is a kind of usual reporter that can emit green fluorescence

We use this part as a verification circuit to verify the comDE system and the PnlmC promotor


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None